GK026 ScienCell 人類Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Wnt Signaling Pathway qPCR Array Kit
ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來(lái)源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。
北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。
GK026 ScienCell人類Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Wnt Signaling Pathway qPCR Array Kit,產(chǎn)品簡(jiǎn)介:
產(chǎn)品品牌; ScienCell
產(chǎn)品貨號(hào):GK026
產(chǎn)品名稱:GK026 ScienCell人類Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Wnt Signaling Pathway qPCR Array Kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
GK026 ScienCell人類Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Wnt Signaling Pathway qPCR Array Kit, 產(chǎn)品說(shuō)明:
ScienCell的GeneQuery™ Human Wnt信號(hào)通路qPCR陣列試劑盒(GQH-WNT)旨在促進(jìn)Wnt信號(hào)轉(zhuǎn)導(dǎo)中涉及的88個(gè)關(guān)鍵基因的基因表達(dá)譜分析。 Wnt信號(hào)通路以其在癌變和胚胎發(fā)育中的作用而聞名。 該試劑盒側(cè)重于規(guī)范的Wnt信號(hào)。 如何根據(jù)目的對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:
配體:所有已知的WNTs。
受體: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6
受體換能器: DVL1, DVL2, DVL3, CSNK1E, MARK1
轉(zhuǎn)錄因子:CTNNB1,TCF7L1,TCF7L2,LEF1,PYGO1
監(jiān)管要素:NLK、NKD1、AES、SFRP1、LRP1
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(i)qPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有說(shuō)明,否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;(iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過(guò)qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。
ScienCell's GeneQuery™ Human Wnt Signaling Pathway qPCR Array Kit (GQH-WNT) is designed to facilitate gene expression profiling of 88 key genes involved in Wnt signaling transduction. The Wnt signaling pathway is well known for its role in carcinogenesis and embryonic development. This kit focuses on canonical Wnt signaling. Brief examples of how included genes may be grouped according to purpose are shown below:
· Ligands: all known WNTs
· Receptors: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6
· Receptor transducers: DVL1, DVL2, DVL3, CSNK1E, MARK1
· Transcription factors: CTNNB1, TCF7L1, TCF7L2, LEF1, PYGO1
· Regulatory elements: NLK, NKD1, AES, SFRP1, LRP1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ 人類 Wnt 信號(hào)通路 qPCR 陣列板布局*(8 個(gè)粗體和斜體對(duì)照)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | AES | CSNK2A1 | DKKL1 | FZD2 | GSK3A | LRP1 | PORCN | RSPO4 | SMO | WNT16 | WNT6 | β-actin |
B | APC | CTNNB1 | DVL1 | FZD3 | GSK3B | LRP5 | PPP2R4 | RYK | TCF7L1 | WNT2 | WNT7A | GAPDH |
C | AXIN1 | CTNNBIP1 | DVL2 | FZD4 | HNF1A | LRP6 | PYGO1 | SFRP1 | TCF7L2 | WNT2B | WNT7B | LDHA |
D | AXIN2 | CUL1 | DVL3 | FZD5 | KREMEN1 | MARK1 | ROR1 | SFRP2 | TLE1 | WNT3 | WNT8A | NONO |
E | BCL9 | DKK1 | FBXW7 | FZD6 | LEF1 | MARK2 | ROR2 | SFRP4 | WNT1 | WNT3A | WNT8B | PPIH |
F | CDC37 | DKK2 | FRZB | FZD7 | LGR4 | MARK3 | RSPO1 | SKP1 | WNT10A | WNT4 | WNT9A | GDC |
G | CSNK1A1L | DKK3 | FZD1 | FZD8 | LGR5 | NKD1 | RSPO2 | SKP2 | WNT10B | WNT5A | WNT9B | PPC |
H | CSNK1E | DKK4 | FZD10 | FZD9 | LGR6 | NLK | RSPO3 | SMARCA4 | WNT11 | WNT5B | ZNRF3 | NTC |
提示:基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新
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