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ScienCellGK029Human Tie2/Angpt Signaling qPCR Array Kit
更新時(shí)間:2023-01-03   點(diǎn)擊次數(shù):485次

GK029 ScienCell Human Tie2/Angpt信號(hào)qPCR檢測(cè)試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來(lái)源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK029 ScienCell Human Tie2/Angpt信號(hào)qPCR檢測(cè)試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號(hào):GK029

產(chǎn)品名稱:GK029 ScienCell Human Tie2/Angpt信號(hào)qPCR檢測(cè)試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

GK029 ScienCell Human Tie2/Angpt信號(hào)qPCR檢測(cè)試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit, 產(chǎn)品說(shuō)明:

ScienCell GeneQuery Human Tie2/Angpt 信號(hào) qPCR 陣列試劑盒 (GQH-TEK) 旨在促進(jìn)參與 Tie2 信號(hào)轉(zhuǎn)導(dǎo)的 88 個(gè)關(guān)鍵基因的基因表達(dá)譜分析,Tie2 信號(hào)轉(zhuǎn)導(dǎo)是血管生成過(guò)程中血管形成的關(guān)鍵組成部分。 如何根據(jù)功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:

配體和受體:ANGPT1,ANGPT2TEK,TIE1

形態(tài): GRB2, PTPN11, SOS1 PTK2, RHOA

動(dòng)力: PLD1 MAP2K1, DOK2, NCK1 CRK

轉(zhuǎn)錄因子:STAT1、STAT5B、MEF2AFOXO3、EGR1

細(xì)胞周期調(diào)節(jié):CDC42,NFKB1,TNIP2,RIPK1,暗黑破壞神

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說(shuō)明,否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過(guò)qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit (GQH-TEK) is designed to facilitate gene expression profiling of 88 key genes involved in Tie2 signaling transduction, which is a key component in the formation of blood vessels during angiogenesis.  Brief examples of how included genes may be grouped according to function are shown below:

·         Ligands & receptors: ANGPT1, ANGPT2, TEK, TIE1

·         Morphology: GRB2, PTPN11, SOS1, PTK2, RHOA  

·         Motility: PLD1, MAP2K1, DOK2, NCK1, CRK

·         Transcription factors: STAT1, STAT5B, MEF2A, FOXO3, EGR1

·         Cell cycle regulation: CDC42, NFKB1, TNIP2, RIPK1, DIABLO

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery Human Tie2/Angpt 信號(hào) qPCR 陣列板布局*8 個(gè)粗體和斜體對(duì)照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ADSS

CDC42

DOK2

FOXO3

HIF1A

ITGB5

MMP2

PDIA3

PTPN11

SELE

TEK

β-actin

B

AKT1

CDKN1A

DOK4

FYN

HRAS

KLF2

MTOR

PGK1

PXN

SELP

TIAM1

GAPDH

C

AKT2

CRK

EGR1

GJA5

ICAM1

MAP2K1

NCK1

PIK3CA

RAC1

SOS1

TIE1

LDHA

D

ALDOA

CTNNB1

ELF2

GRB14

ILK

MAPK1

NFKB1

PIK3R1

RAF1

SPHK1

TLN1

NONO

E

ANGPT1

CXCL12

ELK1

GRB2

ITGA5

MAPK14

NOS3

PIK3R2

RASA1

STAT1

TNIP2

PPIH

F

ANGPT2

DIABLO

EPAS1

GRB7

ITGAV

MAPK3

NOX1

PLCG1

RELA

STAT3

TXNRD1

GDC

G

ARHGAP5

DIAPH1

FES

GYS1

ITGB1

MAPK7

NR4A1

PLD1

RHOA

STAT5A

VCAM1

PPC

H

BIRC5

DLL4

FOXO1

HAGH

ITGB3

MEF2A

PAK1

PTK2

RIPK1

STAT5B

VEGFA

NTC

提示:基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新

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