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ScienCellGK035人體缺氧反應qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):487次

GK035 ScienCell 人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK035 ScienCell人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK035

產(chǎn)品名稱:GK035 ScienCell人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

GK035 ScienCell人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit 產(chǎn)品說明:

缺氧是指與正常有氧條件相比氧氣含量降低的任何環(huán)境。 當細胞缺氧時,會誘導缺氧反應,長時間缺氧通常會導致局部細胞環(huán)境的生理補償,例如缺氧區(qū)域的血管生成增加。 ScienCell GeneQuery™ 人類缺氧反應 qPCR 陣列試劑盒 (GQH-HPX) 旨在促進參與細胞對低氧條件反應的 88 個關鍵基因的基因表達譜分析。 如何根據(jù)反應類型對所包含的基因進行分組的簡要示例如下所示:

細胞代謝: ENO1, HK1 PFKFB3, PGK1, SLC2A1

炎癥: CXCL8 IL1A, CCL2 IL6, THBD

血管松弛:AHSP,PTGIS

細胞死亡或增殖:BNIP3LNOS3、PIM1、EPO

血管生成: KDR, PDGFB, PLAU, TEK FLT1

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions.  The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region.  ScienCell's GeneQuery™ Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions.  Brief examples of how included genes may be grouped according to response type are shown below:

·         Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1

·         Inflammation: CXCL8, IL1A, CCL2, IL6, THBD

·         Vasorelaxation: AHSP, PTGIS

·         Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO

·         Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人內(nèi)皮細胞生物學 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

AHSP

BNIP3L

EGLN1

EPO

HIF3A

JMJD6

OS9

PIK3CA

PTGIR

SAT2

TEK

β-actin

B

ALDOA

CA9

EGLN2

F2

HK1

KDR

PDGFB

PIM1

PTGIS

SELP

THBD

GAPDH

C

ANXA2

CCL2

EGLN3

FLT1

HRH1

LDHA

PDPK1

PLAU

PTGS2

SENP1

TNF

LDHA

D

APEX1

COPS5

EGR1

FOXO4

ICAM1

LGALS3

PECAM1

PPP3CB

RACK1

SLC2A1

TP53

NONO

E

ARNT

CREBBP

EIF4E

GSK3B

IFNG

LTC4S

PER1

PPP3CC

RPS6

SP1

TXN

PPIH

F

ARNT2

CUL2

ENO1

GYS1

IL1A

MAP3K1

PFKFB3

PRKCA

RUVBL2

STAT3

USP20

GDC

G

ARNTL

CXCL8

EP300

HIF1A

IL4

NFKB1

PFKFB4

PSMC6

RWDD3

TCEB1

VCAM1

PPC

H

BNIP3

EDN1

EPAS1

HIF1AN

IL6

NOS3

PGK1

PTGFR

SAT1

TCEB2

VHL

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息:

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