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ScienCellGK072人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒
更新時(shí)間:2023-01-03   點(diǎn)擊次數(shù):422次

GK072 ScienCell 人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Adaptive Immune Response qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來(lái)源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開(kāi)發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK072 ScienCell人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Adaptive Immune Response qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號(hào):GK072

產(chǎn)品名稱:GK072 ScienCell人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Adaptive Immune Response qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK072 ScienCell人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Adaptive Immune Response qPCR Array Kit, 產(chǎn)品說(shuō)明:

ScienCellGeneQuery™人類適應(yīng)性免疫反應(yīng)qPCR陣列(GQH-AIR)有助于對(duì)參與適應(yīng)性免疫或獲得性免疫的88個(gè)關(guān)鍵基因進(jìn)行基因表達(dá)分析。 脊椎動(dòng)物有兩個(gè)主要的免疫系統(tǒng):先天性和適應(yīng)性。 雖然先天免疫系統(tǒng)通常負(fù)責(zé)宿主防御病原體,但適應(yīng)性免疫系統(tǒng)負(fù)責(zé)防御特定病原體,從而賦予免疫記憶。 如何根據(jù)其在適應(yīng)性免疫反應(yīng)中的功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:

抗原呈遞:CANX、HLA-A、HLA-DPB1PSMB8、TAP2

Th1標(biāo)志物:IL12RB1,CCR5,CD94CXCR3。IL18R

Th2 標(biāo)志物:IL13IL1R1、IL4CCR8、CSF2

Th17 標(biāo)記: CCR6, IL17A, IL6 RORC, IRF4

Treg 標(biāo)記物:FOXP3、CD4、IL10、IL2RA、CTLA4

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可識(shí)別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說(shuō)明,否則引物集可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過(guò)qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery Human Adaptive Immune Response qPCR Array (GQH-AIR) facilitates gene expression profiling of 88 key genes involved in adaptive immunity or acquired immunity.  Vertebrates have two main immune systems: innate and adaptive.  While the innate immune system is responsible for host defense against pathogens in a general way, the adaptive immune system is responsible for defense against particular pathogens, thereby conferring immunological memory.  Brief examples of how included genes may be grouped according to their function in the adaptive immune response are shown below:

·         Antigen presentation: CANX, HLA-A, HLA-DPB1, PSMB8, TAP2

·         Th1 markers: IL12RB1, CCR5, CD94, CXCR3. IL18R

·         Th2 markers: IL13, IL1R1, IL4, CCR8, CSF2

·         Th17 markers: CCR6, IL17A, IL6, RORC, IRF4

·         Treg markers: FOXP3, CD4, IL10, IL2RA, CTLA4

GeneQuery qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類適應(yīng)性免疫應(yīng)答 qPCR 陣列板布局*8 個(gè)粗體和斜體對(duì)照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC1

CCR8

CD40LG

FOXP3

HLA-DQA1

IL12RB1

IL1R1

IRF4

NOTCH3

PTPRC

STAT6

β-actin

B

ARG1

CD19

CD80

GATA3

HLA-DQB1

IL12RB2

IL1R2

ITGAM

PDIA3

RAG1

TAP2

GAPDH

C

CANX

CD27

CD86

HAVCR2

HLA-DRA

IL13

IL2

JAK3

PSMB8

RAG2

TBX21

LDHA

D

CCL5

CD28

CSF2

HLA-A

HLA-DRB1

IL17A

IL2RA

KLRD1

PSMB9

RORA

TNF

NONO

E

CCR1

CD3D

CTLA4

HLA-B

HLA-DRB3

IL17B

IL4

LGALS1

PSME1

RORC

TNFRSF4

PPIH

F

CCR4

CD3E

CXCR3

HLA-C

IFNG

IL17C

IL5

LTA

PSME2

STAT1

TNFSF11

GDC

G

CCR5

CD3G

CXCR4

HLA-DPA1

IL10

IL18

IL6

MS4A1

PSME3

STAT3

TRAT1

PPC

H

CCR6

CD4

CXCR6

HLA-DPB1

IL12A

IL18R1

IL9

NFKB1

PTGDR2

STAT4

ZAP70

NTC

提示:基因選擇可能會(huì)根據(jù)新的研究和開(kāi)發(fā)進(jìn)行更新

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GK006   人類神經(jīng)發(fā)育和再生qPCR檢測(cè)試劑盒,Human Neural Development and Regeneration qPCR Array Kit

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GK009   人類星型膠質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細(xì)胞發(fā)育和色素沉著qPCR檢測(cè)試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

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GK020   B細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

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GK022   人內(nèi)吞作用qPCR檢測(cè)試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內(nèi)細(xì)胞分化qPCR檢測(cè)試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應(yīng)力和機(jī)械傳導(dǎo)qPCR檢測(cè)試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號(hào)靶基因qPCR檢測(cè)試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號(hào)qPCR檢測(cè)試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測(cè)試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應(yīng)qPCR檢測(cè)試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號(hào)響應(yīng)qPCR檢測(cè)試劑盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬細(xì)胞細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人類吞噬作用qPCR檢測(cè)試劑盒,Human Phagocytosis qPCR Array Kit

GK039   人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纖維細(xì)胞生長(zhǎng)因子信號(hào)通路qPCR檢測(cè)試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測(cè)試劑盒,Human Skin Wound Healing qPCR Array Kit

GK056   人體止血qPCR檢測(cè)試劑盒,Human Hemostasis qPCR Array Kit

GK060   人類晝夜節(jié)律qPCR檢測(cè)試劑盒,Human Circadian Rhythm qPCR Array Kit

GK061   人類DNA損傷感應(yīng)qPCR檢測(cè)試劑盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人類細(xì)胞生長(zhǎng)和分裂qPCR檢測(cè)試劑盒,Human Cell Growth and Division qPCR Array Kit

GK063   人細(xì)胞周期蛋白和細(xì)胞周期蛋白依賴性激酶qPCR檢測(cè)試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人類小膠質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人類小膠質(zhì)細(xì)胞Sensome qPCR檢測(cè)試劑盒,Human Microglial Sensome qPCR Array Kit

GK074   人角質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Keratocyte Biology qPCR Array

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GK089   人類Ephrin信號(hào)qPCR檢測(cè)試劑盒,Human Ephrin Signaling qPCR Array Kit

GK090   人體細(xì)胞連接qPCR檢測(cè)試劑盒,Human Cell Junctions qPCR Array Kit

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GK092   人胰腺星狀細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR ArrayHuman Mesenchymal Stem Cell Differentiation qPCR Array Kit

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GK105   人類ECM降解qPCR檢測(cè)試劑盒,Human ECM Degradation qPCR Array Kit

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GK114   人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒,Human Microglial Polarization Markers qPCR Array Kit

GK115   人類骨髓譜系標(biāo)記qPCR檢測(cè)試劑盒,Human Myeloid Lineage Markers qPCR Array Kit

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GK117   人胸腺細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Thymocyte Development Markers qPCR Array Kit

GK118   人類先天淋巴細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119   人類神經(jīng)祖細(xì)胞標(biāo)記qPCR檢測(cè)試劑盒,Human Neural Progenitor Markers qPCR Array Kit

GK121   PD-1/PD-L1檢查點(diǎn)途徑qPCR檢測(cè)試劑盒,GeneQuery Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit

GK123   人類生長(zhǎng)因子qPCR檢測(cè)試劑盒,Human Growth Factors qPCR Array Kit

GK124   人類轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Transcription Factors qPCR Array Kit

GK125   人干細(xì)胞轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細(xì)胞表面標(biāo)記qPCR檢測(cè)試劑盒,Human Cell Surface Markers qPCR Array Kit

GK127   人腦發(fā)育標(biāo)記物qPCR檢測(cè)試劑盒,Human Brain Development Markers qPCR Array Kit



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