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ScienCellGK073人類小膠質(zhì)細(xì)胞Sensome
更新時間:2023-01-03   點擊次數(shù):508次

GK073 ScienCell 人類小膠質(zhì)細(xì)胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK073 ScienCell人類小膠質(zhì)細(xì)胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK073

產(chǎn)品名稱:GK073 ScienCell人類小膠質(zhì)細(xì)胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK073 ScienCell人類小膠質(zhì)細(xì)胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit, 產(chǎn)品說明:

ScienCellGeneQuery™人小膠質(zhì)細(xì)胞SensomeqPCR陣列(GQH-MGS)有助于對88個關(guān)鍵基因進(jìn)行基因表達(dá)分析,這些基因涉及小膠質(zhì)細(xì)胞如何感知其環(huán)境。 小膠質(zhì)細(xì)胞被認(rèn)為是“中樞神經(jīng)系統(tǒng)的常駐巨噬細(xì)胞",因此它們?nèi)绾胃兄h(huán)境對于在病原體入侵、損傷或變性期間維持大腦中的穩(wěn)態(tài)功能是bukehuoque的。 該特定陣列中包含的基因側(cè)重于在衰老過程中具有dute差異表達(dá)的基因。 如何根據(jù)其功能對所包含的基因進(jìn)行分組的示例如下所示:

經(jīng)典灌注:PTGS2、TNFCXCL10

替代引發(fā):SPP1,馬可,CXCR4,CLEC7A,CD163

趨化因子受體:PTAFR,CMKLR1,GPR77,C5AR1,CCR5

嘌呤能受體:TMEM173,P2RY1,P2RY12,GPR34ADORA3

老化: TREM2, TLR8, SCARA3, IFITM3, AGER

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。

ScienCell's GeneQuery Human Microglial Sensome qPCR Array (GQH-MGS) facilitates gene expression profiling of 88 key genes involved in how microglia perceive their environment.  Microglia are considered to be the resident macrophages of the CNS," so how they sense their environment is integral to maintaining homeostatic functions in the brain during pathogen invasion, injury, or degeneration.  The genes included in this particular array focus on genes with unique differential expression in aging.  Examples of how included genes may be grouped according to their function are shown below:

·         Classical priming: PTGS2, TNF, CXCL10

·         Alternative priming: SPP1, MARCO, CXCR4, CLEC7A, CD163

·         Chemokine receptors: PTAFR, CMKLR1, GPR77, C5AR1, CCR5

·         Purinergic receptors: TMEM173, P2RY1, P2RY12, GPR34, ADORA3

·         Aging: TREM2, TLR8, SCARA3, IFITM3, AGER

GeneQuery qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人小膠質(zhì)細(xì)胞森納體 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ADORA3

CCL8

CD22

CD86

CXCR2

FCGR2B

HAVCR2

ITGB2

P2RX5

SELPLG

TLR2

β-actin

B

AGER

CCR1

CD33

CLEC5A

CXCR4

FCGR3A

IFITM1

LAIR1

P2RY1

SIGLEC1

TLR7

GAPDH

C

ALOX15

CCR2

CD37

CLEC7A

CYSLTR1

FCRL1

IFITM2

LGALS9

P2RY12

SIGLEC5

TLR8

LDHA

D

ARG1

CCR5

CD48

CMKLR1

ECSCR

FPR2

IFITM3

LTF

P2RY13

SLC2A5

TMEM173

NONO

E

C3AR1

CCRL2

CD52

CMTM7

F13A1

GAS7

IFITM5

LY86

PTAFR

SLC7A7

TNF

PPIH

F

C5AR1

CD14

CD68

CSF1R

FCER1A

GPR183

IL10RA

MARCO

PTGS2

SLCO2B1

TNFRSF1B

GDC

G

C5AR2

CD163

CD74

CX3CR1

FCER1G

GPR34

IL1RN

NRG1

PTPRC

SPP1

TREM2

PPC

H

CCL20

CD180

CD84

CXCL10

FCGR1A

GPR84

ITGAM

OLR1

SCARA3

TGFBR1

TYROBP

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息:

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GK003   人類細(xì)胞周期qPCR檢測試劑盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運(yùn)qPCR檢測試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人類星型膠質(zhì)細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細(xì)胞發(fā)育和色素沉著qPCR檢測試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質(zhì)細(xì)胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內(nèi)皮細(xì)胞表型異質(zhì)性qPCR檢測試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內(nèi)皮細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細(xì)胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細(xì)胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內(nèi)細(xì)胞分化qPCR檢測試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應(yīng)力和機(jī)械傳導(dǎo)qPCR檢測試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號通路qPCR檢測試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應(yīng)qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號響應(yīng)qPCR檢測試劑盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬細(xì)胞細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人類吞噬作用qPCR檢測試劑盒,Human Phagocytosis qPCR Array Kit

GK039   人類先天免疫反應(yīng)qPCR檢測試劑盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纖維細(xì)胞生長因子信號通路qPCR檢測試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測試劑盒,Human Skin Wound Healing qPCR Array Kit

GK056   人體止血qPCR檢測試劑盒,Human Hemostasis qPCR Array Kit

GK060   人類晝夜節(jié)律qPCR檢測試劑盒,Human Circadian Rhythm qPCR Array Kit

GK061   人類DNA損傷感應(yīng)qPCR檢測試劑盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人類細(xì)胞生長和分裂qPCR檢測試劑盒,Human Cell Growth and Division qPCR Array Kit

GK063   人細(xì)胞周期蛋白和細(xì)胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人類小膠質(zhì)細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人類適應(yīng)性免疫反應(yīng)qPCR檢測試劑盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人類小膠質(zhì)細(xì)胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

GK074   人角質(zhì)細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人類周細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

GK084   人體軸突導(dǎo)向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

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GK088   VEGF信號通路qPCR檢測試劑盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人類Ephrin信號qPCR檢測試劑盒,Human Ephrin Signaling qPCR Array Kit

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GK093   Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR ArrayHuman Mesenchymal Stem Cell Differentiation qPCR Array Kit

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GK114   人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測試劑盒,Human Microglial Polarization Markers qPCR Array Kit

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GK126   人類細(xì)胞表面標(biāo)記qPCR檢測試劑盒,Human Cell Surface Markers qPCR Array Kit

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