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ScienCellGK095人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測的特點
更新時間:2023-01-10   點擊次數(shù):615次

GK095 ScienCell 人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務(wù)標準。

GK095 ScienCell人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK095

產(chǎn)品名稱:GK095 ScienCell人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

北京裕恒豐科技有限公司是ScienCell中國區(qū)總代理,提供各種基因表達分析相關(guān)品,ScienCell細胞培養(yǎng)基、轉(zhuǎn)染試劑、細胞檢測試劑盒等細胞生物學產(chǎn)品。

GK095 ScienCell人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人間充質(zhì)到上皮轉(zhuǎn)化 qPCR 陣列試劑盒 (GQH-MET) 旨在促進參與可逆間充質(zhì)到上皮轉(zhuǎn)化 (MET) 的 88 個關(guān)鍵基因的基因表達譜分析。MET和反向過程,上皮到間充質(zhì)轉(zhuǎn)化(EMT),參與胚胎發(fā)生,癌癥轉(zhuǎn)移和干細胞重編程。下面顯示了如何對基因進行分組的簡要示例:    

EMT標記: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1 ETS1, FOXC2, GSC, PDGFD

EMT-MET過渡:PAX2,WT1BMP7,TWIST1PRRX1

增殖和形態(tài)發(fā)生控制:AKT1,BMP1BMP7,EGFR,FGFBP1IGFBP4,JAG1PDGFRB,SNAI1SOX10,VCAN,WNT5A,WNT11,ZEB1

分化控制: BMP2 COL3A1, ERBB3 F11R, FZD7, GSC JAG1, KRT14, 節(jié)點, SMAD2, SOX10 WNT5B

調(diào)節(jié) MET 的轉(zhuǎn)錄因子:CTNNB1ESR1、FOXC2NOTCH1、SMAD2、SNAI1/2/3、STAT3TCF3、TCF4、TWIST1、ZEB2

MET中涉及的信號通路

雌激素受體:CAV2,ESR1,KRT19,TGFB3

GPCR AKT1FZD7, GNG11, RAC1 RGS2

PI3K/AKT AKT1, AKT2, PIK3CA PIK3CD, PIK3CG PIK3R1, PIK3R3, PIK3R5

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:    

·         EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD

·         EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1

·         Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1

·         Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B

·         Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2

·         Signaling pathways involved in MET

o    Estrogen receptor: CAV2, ESR1, KRT19, TGFB3

o    GPCR: AKT1, FZD7, GNG11, RAC1, RGS2

o    PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人間充質(zhì)到上皮過渡 qPCR 陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CAV2

COL3A1

F11R

IGFBP4

MUC1

PIK3CG

RGS2

SNAI3

TGFB2

VCAN

β-actin

B

AKT1

CDH1

COL5A2

FGFBP1

ILK

NODAL

PIK3R1

RPS27A

SOX10

TGFB3

VIM

GAPDH

C

AKT2

CDH11

CTNNB1

FN1

JAG1

NOTCH1

PIK3R3

S100A4

SPINT2

TGFBR1

WNT11

LDHA

D

ARHGEF18

CDH2

DSP

FOXC2

KRT14

PAX2

PIK3R5

SDC1

STAT3

TGFBR2

WNT5A

NONO

E

AXL

CDKN2A

EGFR

FZD7

KRT19

PDGFD

PRKCZ

SMAD2

STK11

TIMP1

WNT5B

PPIH

F

BMP1

CDKN2B

ERBB3

GNG11

LEF1

PDGFRB

PRRX1

SMURF1

TCF3

TRIM28

WT1

GDC

G

BMP2

CLDN4

ESR1

GSC

MMP2

PIK3CA

RAB25

SNAI1

TCF4

TWIST1

ZEB1

PPC

H

BMP7

CLDN7

ETS1

HNRNPAB

MST1R

PIK3CD

RAC1

SNAI2

TGFB1

UBA52

ZEB2

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息:

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GK002   人類多能干細胞qPCR檢測試劑盒,Human Pluripotent Stem Cell qPCR Array Kit

GK003   人類細胞周期qPCR檢測試劑盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮細胞生物學qPCR檢測試劑盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運qPCR檢測試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人類星型膠質(zhì)細胞生物學qPCR檢測試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細胞發(fā)育和色素沉著qPCR檢測試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細胞生物學qPCR檢測試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質(zhì)細胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內(nèi)皮細胞表型異質(zhì)性qPCR檢測試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內(nèi)皮細胞生物學qPCR檢測試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內(nèi)細胞分化qPCR檢測試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應力和機械傳導qPCR檢測試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號通路qPCR檢測試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號響應qPCR檢測試劑盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬細胞細胞生物學qPCR檢測試劑盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人類吞噬作用qPCR檢測試劑盒,Human Phagocytosis qPCR Array Kit

GK039   人類先天免疫反應qPCR檢測試劑盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纖維細胞生長因子信號通路qPCR檢測試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測試劑盒,Human Skin Wound Healing qPCR Array Kit

GK056   人體止血qPCR檢測試劑盒,Human Hemostasis qPCR Array Kit

GK060   人類晝夜節(jié)律qPCR檢測試劑盒,Human Circadian Rhythm qPCR Array Kit

GK061   人類DNA損傷感應qPCR檢測試劑盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人類細胞生長和分裂qPCR檢測試劑盒,Human Cell Growth and Division qPCR Array Kit

GK063   人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人類小膠質(zhì)細胞生物學qPCR檢測試劑盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人類適應性免疫反應qPCR檢測試劑盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

GK074   人角質(zhì)細胞生物學qPCR檢測試劑盒,Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細胞生物學qPCR檢測試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細胞生物學qPCR檢測試劑盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人類周細胞生物學qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

GK084   人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

GK087   人類染色質(zhì)組織和重塑qPCR檢測試劑盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088   VEGF信號通路qPCR檢測試劑盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人類Ephrin信號qPCR檢測試劑盒,Human Ephrin Signaling qPCR Array Kit

GK090   人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit

GK091   人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人類雪旺細胞生物學qPCR檢測試劑盒,Human Schwann Cell Biology qPCR Array Kit

GK097   人類平滑肌細胞生物學qPCR檢測試劑盒,Human Smooth Muscle Cell Biology qPCR Array Kit

GK098   人類心臟肌細胞生物學qPCR檢測試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit

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GK105   人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit

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GK111   人凝集素調(diào)節(jié)免疫應答qPCR檢測試劑盒,Human Lectin Regulation in Immune Response qPCR Array Kit

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GK113   人類神經(jīng)干細胞標記qPCR檢測試劑盒,Human Neural Stem Cell Markers qPCR Array Kit

GK114   人類小膠質(zhì)細胞極化標記qPCR檢測試劑盒,Human Microglial Polarization Markers qPCR Array Kit

GK115   人類骨髓譜系標記qPCR檢測試劑盒,Human Myeloid Lineage Markers qPCR Array Kit

GK116   B細胞和T細胞發(fā)育標記qPCR檢測試劑盒,Human B and T Cells Development Markers qPCR Array Kit

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GK118   人類先天淋巴細胞發(fā)育標記qPCR檢測試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119   人類神經(jīng)祖細胞標記qPCR檢測試劑盒,Human Neural Progenitor Markers qPCR Array Kit

GK121   PD-1/PD-L1檢查點途徑qPCR檢測試劑盒,GeneQuery Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit

GK123   人類生長因子qPCR檢測試劑盒,Human Growth Factors qPCR Array Kit

GK124   人類轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Transcription Factors qPCR Array Kit

GK125   人干細胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細胞表面標記qPCR檢測試劑盒,Human Cell Surface Markers qPCR Array Kit

GK127   人腦發(fā)育標記物qPCR檢測試劑盒,Human Brain Development Markers qPCR Array Kit



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