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ScienCellGK098人類心臟肌細胞生物學qPCR檢測的性能
更新時間:2023-01-10   點擊次數(shù):754次

GK098 ScienCell 人類心臟肌細胞生物學qPCR檢測試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK098 ScienCell人類心臟肌細胞生物學qPCR檢測試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK098

產(chǎn)品名稱:GK098 ScienCell人類心臟肌細胞生物學qPCR檢測試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

北京裕恒豐科技有限公司是ScienCell中國區(qū)總代理,提供各種基因表達分析相關品,ScienCell細胞培養(yǎng)基、轉染試劑、細胞檢測試劑盒等細胞生物學產(chǎn)品。

GK098 ScienCell人類心臟肌細胞生物學qPCR檢測試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人心肌細胞生物學 qPCR 陣列試劑盒 (GQH-CMB) 旨在促進參與人類心肌細胞生物學的 88 個關鍵基因的基因表達譜分析,例如心肌收縮、心肌細胞增殖和分化以及心臟 z 盤信號網(wǎng)絡。與選定的心肌細胞相關疾病有關的基因也包含在陣列中。下面顯示了如何根據(jù)其功能對基因進行分組的簡要示例:    

心肌細胞標志物:ACTN1、TNNT2NKX2-5、GJA1NPPA、MYH6MYH7B、TNNI3CAV3、GATA4MYL2、MEF2C

心肌收縮: CAMK2s ATP2As, MYH7, MYL2, CACNA1C, HRC, ITPR3, KCNJ5 TRDN, TPM

心臟Z盤信號網(wǎng)絡:AGTR1ACTN,CSRP3,MYOZ2,ITGB1BP2,DESITGA1,ITGB1ILK,CACNA1s,CIB1MYOZ2/3

心肌細胞發(fā)育和增殖:GATA4,HAND2MEF2C,TBX5,FOXP1,NKX2-5,FGFsPIK3CB,SAV1STK3 / 4,YAP1WWTR1

心肌細胞分化:肌細胞分化,TWIST1,VANGL2,BCKDHACPT1B,GPAM,POU5F1MEF2C,NPPA/B,MYOG

與以下因素有關的基因

肥厚型心肌病: MYH6/7, MYBPC3, TNNI3, PMPCA, TNNT2, TPM1, ACTC1 MYL2/3

心肌梗死: OLR1, LRP8, LTA LGALS2, ESR1, FABP6

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Cardiac Myocyte Biology qPCR Array Kit (GQH-CMB) is designed to facilitate gene expression profiling of 88 key genes involved in human cardiac myocyte biology, such as cardiac muscle contraction, cardiac myocyte proliferation and differentiation, and cardiac z-disk signaling network. Genes implicated in selected cardiac myocyte related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:    

·         Cardiac myocyte markers: ACTN1, TNNT2, NKX2-5, GJA1, NPPA, MYH6, MYH7B, TNNI3, CAV3, GATA4, MYL2, MEF2C

·         Cardiac muscle contraction: CAMK2s, ATP2As, MYH7, MYL2, CACNA1C, HRC, ITPR3, KCNJ5, TRDN, TPMs

·         Cardiac z-disk signaling network: AGTR1, ACTNs, CSRP3, MYOZ2, ITGB1BP2, DES, ITGA1, ITGB1, ILK, CACNA1s, CIB1, MYOZ2/3

·         Cardiac myocyte development and proliferation: GATA4, HAND2, MEF2C, TBX5, FOXP1, NKX2-5, FGFs, PIK3CB, SAV1, STK3/4, YAP1, WWTR1

·         Cardiac myocyte differentiation: MYOCD, TWIST1, VANGL2, BCKDHA, CPT1B, GPAM, POU5F1, MEF2C, NPPA/B, MYOG

·         Genes implicated in

o    hypertrophic cardiomyopathy: MYH6/7, MYBPC3, TNNI3, PMPCA, TNNT2, TPM1, ACTC1, MYL2/3

o    myocardial infarction: OLR1, LRP8, LTA, LGALS2, ESR1, FABP6

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人心肌細胞生物學 qPCR 陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTN1

ATP2A3

CAMK2D

FABP6

GLRX3

ITPR3

MYBPC3

MYOZ2

PMPCA

SLC9A1

TPM2

β-actin

B

ACTN2

BCKDHA

CAMK2G

FGF10

GPAM

KAT2B

MYH6

MYOZ3

POU5F1

STK3

TPM3

GAPDH

C

ACTC1

CACNA1C

CAV3

FGF16

HAND2

KCNJ5

MYH7

NKX2-5

PRKCB

STK4

TPM4

LDHA

D

ACTN3

CACNA1D

CIB1

FGF20

HRC

LGALS2

MYH7B

NPPA

QDPR

SYNPO2

TRDN

NONO

E

ACTN4

CACNA1F

CPT1B

FGF3

ILK

LMCD1

MYL2

NPPB

RYR2

TBX5

TWIST1

PPIH

F

AGTR1

CACNA1S

CSRP3

FOXP1

ITGA1

LRP8

MYL3

OLR1

SAV1

TNNI3

VANGL2

GDC

G

ATP2A1

CAMK2A

DES

GATA4

ITGB1

LTA

MYOCD

PAK1

SCN5A

TNNT2

WWTR1

PPC

H

ATP2A2

CAMK2B

ESR1

GJA1

ITGB1BP2

MEF2C

MYOG

PIK3CB

SLC8A1

TPM1

YAP1

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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