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ScienCell GK105人類ECM降解qPCR檢測試劑盒簡介
更新時間:2023-01-04   點擊次數(shù):527次

GK105 ScienCell 人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK105 ScienCell人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK105

產(chǎn)品名稱:GK105 ScienCell人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

 

GK105 ScienCell人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit 產(chǎn)品說明:

ScienCell GeneQuery™ 人類 ECM 降解 qPCR 陣列試劑盒 (GQH-ECM) 分析了參與細胞外基質(zhì) (ECM) 降解的 88 個關(guān)鍵基因。 ECM由提供結(jié)構(gòu)和生化支持的細胞分泌的分子組成。 它的作用包括支持細胞間通訊、生長因子儲存以及調(diào)節(jié)細胞遷移和運動。 因此,ECM的降解會影響生長因子釋放,趨化性,細胞遷移和細胞粘附。 通常參與 ECM 降解的酶包括絲氨酸蛋白酶、蘇氨酸蛋白酶和基質(zhì)金屬蛋白酶。 如何對所包含的基因進行分類的簡要示例如下所示:

ECM組件:ACAN,COL18A1,COL4A1FGG,LAMA5,NTN4

蛋白酶: ADAM10, ADAMTS1, BMP2, CAPN9, CTSG, MMP2, MMP9

蛋白酶抑制劑:A2M、CAST、SERPINH1、TIMP1、TIMP2

ECM蛋白聚糖:BGNDCN,LTBP2,LUM,PCOLCE

細胞粘附受體和糖蛋白:CD44,CEACAM8FN1HSPG2,SPARC

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human ECM Degradation qPCR Array Kit (GQH-ECM) profiles 88 key genes involved in extracellular matrix (ECM) degradation.  The ECM comprises molecules secreted by cells that provide structural and biochemical support.  Its roles include supporting intercellular communication, growth factor storage, and regulating cell migration and motility.  Degradation of the ECM thus influences growth factor release, chemotaxis, cell migration, and cellular adhesion.  Enzymes classically involved in ECM degradation include serine proteases, threonine proteases, and matrix metalloproteinases.  Brief examples of how included genes may be categorized are shown below:

·         ECM components: ACAN, COL18A1, COL4A1, FGG, LAMA5, NTN4

·         Proteases: ADAM10, ADAMTS1, BMP2, CAPN9, CTSG, MMP2, MMP9

·         Protease inhibitors: A2M, CAST, SERPINH1, TIMP1, TIMP2

·         ECM proteoglycans: BGN, DCN, LTBP2, LUM, PCOLCE

·         Cell adhesion receptors and glycoproteins: CD44, CEACAM8, FN1, HSPG2, SPARC

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

 

基因查詢™人類 ECM 降解 qPCR 陣列板布局*8 個粗體和斜體對照)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

A2M

ADAMTS4

CAPN1

CEACAM8

COL4A1

CTSG

FGB

LAMA2

LOX

MMP3

PLG

β-actin

B

ACAN

ADAMTS5

CAPN10

COL11A1

COL4A3

CTSS

FGG

LAMA3

LTBP2

MMP7

PSEN1

GAPDH

C

ADAM10

BGN

CAPN2

COL14A1

COL4A5

CTSV

FN1

LAMA5

LUM

MMP8

SERPINH1

LDHA

D

ADAM12

BMP1

CAPN3

COL18A1

COL5A1

DCN

HSPG2

LAMB1

MMP1

MMP9

SPARC

NONO

E

ADAM15

BMP10

CAPN9

COL1A1

COL5A2

DMD

HTRA1

LAMB2

MMP12

NCSTN

SPP1

PPIH

F

ADAM17

BMP2

CASP3

COL1A2

COL6A1

DMP1

KLK12

LAMB3

MMP13

NID1

TIMP1

GDC

G

ADAM8

BMP4

CAST

COL2A1

COL6A3

FBN1

KLK7

LAMC1

MMP14

NTN4

TIMP2

PPC

H

ADAMTS1

BMP7

CD44

COL3A1

COL7A1

FGA

LAMA1

LAMC2

MMP2

PCOLCE

VCAN

NTC

 

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

 

ScienCell基因表達分析產(chǎn)品訂購信息:

GK001   人類細胞凋亡qPCR檢測試劑盒,Human Apoptosis qPCR Array Kit

GK002   人類多能干細胞qPCR檢測試劑盒,Human Pluripotent Stem Cell qPCR Array Kit

GK003   人類細胞周期qPCR檢測試劑盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮細胞生物學qPCR檢測試劑盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運qPCR檢測試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人類星型膠質(zhì)細胞生物學qPCR檢測試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細胞發(fā)育和色素沉著qPCR檢測試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細胞生物學qPCR檢測試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質(zhì)細胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內(nèi)皮細胞表型異質(zhì)性qPCR檢測試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內(nèi)皮細胞生物學qPCR檢測試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內(nèi)細胞分化qPCR檢測試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應力和機械傳導qPCR檢測試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號通路qPCR檢測試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號響應qPCR檢測試劑盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬細胞細胞生物學qPCR檢測試劑盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人類吞噬作用qPCR檢測試劑盒,Human Phagocytosis qPCR Array Kit

GK039   人類先天免疫反應qPCR檢測試劑盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纖維細胞生長因子信號通路qPCR檢測試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測試劑盒,Human Skin Wound Healing qPCR Array Kit

GK056   人體止血qPCR檢測試劑盒,Human Hemostasis qPCR Array Kit

GK060   人類晝夜節(jié)律qPCR檢測試劑盒,Human Circadian Rhythm qPCR Array Kit

GK061   人類DNA損傷感應qPCR檢測試劑盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人類細胞生長和分裂qPCR檢測試劑盒,Human Cell Growth and Division qPCR Array Kit

GK063   人細胞周期蛋白和細胞周期蛋白依賴性激酶qPCR檢測試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人類小膠質(zhì)細胞生物學qPCR檢測試劑盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人類適應性免疫反應qPCR檢測試劑盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒,Human Microglial Sensome qPCR Array Kit

GK074   人角質(zhì)細胞生物學qPCR檢測試劑盒,Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細胞生物學qPCR檢測試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細胞生物學qPCR檢測試劑盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人類周細胞生物學qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

GK084   人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

GK087   人類染色質(zhì)組織和重塑qPCR檢測試劑盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088   VEGF信號通路qPCR檢測試劑盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人類Ephrin信號qPCR檢測試劑盒,Human Ephrin Signaling qPCR Array Kit

GK090   人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit

GK091   人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR ArrayHuman Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人間充質(zhì)至上皮細胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人類雪旺細胞生物學qPCR檢測試劑盒,Human Schwann Cell Biology qPCR Array Kit

GK097   人類平滑肌細胞生物學qPCR檢測試劑盒,Human Smooth Muscle Cell Biology qPCR Array Kit

GK098   人類心臟肌細胞生物學qPCR檢測試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit

GK099   人類骨骼肌細胞生物學qPCR檢測試劑盒,Human Skeletal Muscle Cell Biology qPCR Array Kit

GK100   人角質(zhì)形成細胞生物學qPCR檢測試劑盒,Human Keratinocyte Cell Biology qPCR Array Kit

GK101   人類Synoviocyte細胞生物學qPCR檢測試劑盒,Human Synoviocyte Cell Biology qPCR Array Kit

GK102   人類滋養(yǎng)層細胞生物學qPCR檢測試劑盒,Human Trophoblast Cell Biology qPCR Array Kit

GK103   人前脂肪細胞生物學qPCR檢測試劑盒,Human Preadipocyte Cell Biology qPCR Array Kit

GK104   人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit

GK105   人類ECM降解qPCR檢測試劑盒,Human ECM Degradation qPCR Array Kit

GK106   人類焦點粘附復合物qPCR檢測試劑盒,Human Focal Adhesion Complexes qPCR Array Kit

GK107   人內(nèi)皮素信號qPCR檢測試劑盒,Human Endothelin Signaling qPCR Array Kit

GK111   人凝集素調(diào)節(jié)免疫應答qPCR檢測試劑盒,Human Lectin Regulation in Immune Response qPCR Array Kit

GK112   人類神經(jīng)分化標記qPCR檢測試劑盒,Human Neural Differentiation Markers qPCR Array Kit

GK113   人類神經(jīng)干細胞標記qPCR檢測試劑盒,Human Neural Stem Cell Markers qPCR Array Kit

GK114   人類小膠質(zhì)細胞極化標記qPCR檢測試劑盒,Human Microglial Polarization Markers qPCR Array Kit

GK115   人類骨髓譜系標記qPCR檢測試劑盒,Human Myeloid Lineage Markers qPCR Array Kit

GK116   B細胞和T細胞發(fā)育標記qPCR檢測試劑盒,Human B and T Cells Development Markers qPCR Array Kit

GK117   人胸腺細胞發(fā)育標記qPCR檢測試劑盒,Human Thymocyte Development Markers qPCR Array Kit

GK118   人類先天淋巴細胞發(fā)育標記qPCR檢測試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119   人類神經(jīng)祖細胞標記qPCR檢測試劑盒,Human Neural Progenitor Markers qPCR Array Kit

GK121   PD-1/PD-L1檢查點途徑qPCR檢測試劑盒,GeneQuery Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit

GK123   人類生長因子qPCR檢測試劑盒,Human Growth Factors qPCR Array Kit

GK124   人類轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Transcription Factors qPCR Array Kit

GK125   人干細胞轉(zhuǎn)錄因子qPCR檢測試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細胞表面標記qPCR檢測試劑盒,Human Cell Surface Markers qPCR Array Kit

GK127   人腦發(fā)育標記物qPCR檢測試劑盒,Human Brain Development Markers qPCR Array Kit

 

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