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ScienCellGK114人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒簡(jiǎn)介
更新時(shí)間:2023-01-06   點(diǎn)擊次數(shù):680次

GK114 ScienCell 人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒,Human Microglial Polarization Markers qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動(dòng)物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國(guó)加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK114 ScienCell人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒,Human Microglial Polarization Markers qPCR Array Kit,產(chǎn)品簡(jiǎn)介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號(hào):GK114

產(chǎn)品名稱:GK114 ScienCell人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒,Human Microglial Polarization Markers qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

 

 

GK114 ScienCell人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒,Human Microglial Polarization Markers qPCR Array Kit, 產(chǎn)品說明:

ScienCellGeneQuery™人小膠質(zhì)細(xì)胞偏振標(biāo)記qPCR陣列試劑盒(GQH-MPM)旨在促進(jìn)參與小膠質(zhì)細(xì)胞極化的40個(gè)標(biāo)記基因的基因表達(dá)譜分析,這對(duì)調(diào)節(jié)先天免疫功能具有重要意義。當(dāng)小膠質(zhì)細(xì)胞受到刺激時(shí),可以觀察到兩種潛在的表型:促炎M1小膠質(zhì)細(xì)胞和抗炎M2小膠質(zhì)細(xì)胞。M1表型通過釋放細(xì)胞因子和撤銷急性炎癥來保護(hù)組織免受入侵病原體的侵害,而M2表型的作用是恢復(fù)組織穩(wěn)態(tài)并刺激組織修復(fù)。如何根據(jù)其功能對(duì)所包含的基因進(jìn)行分組的簡(jiǎn)要示例如下所示:   

M1 表型標(biāo)記物:TNF、FCGR2A、FCGR2BFCGR2C、FCGR3A、FCGR3B、CD86、IL1B、NOS2IL6

M2 表型標(biāo)記:MRC1、IL4、IL10、TGFB1、IL1R1、SOCS3、ARG1、IL13、SPHK1

激活/靜息標(biāo)志物:CD80,CLEC5A,HEXB,HLA-DQA1,HLA-DQB1,HLA-DRA,HLA-DRB1IRF8,ITGAMMYB,PTGES,RUNX1,TLR3,TLR4

其他小膠質(zhì)細(xì)胞標(biāo)志物:ADGRE1AIF1,CD40,CD68,EDA,ITGAMITGAX,PTPRC

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個(gè)孔包含一個(gè)引物組,可以特異性識(shí)別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識(shí)別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個(gè)基因被擴(kuò)增。每個(gè)引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Microglial Polarization Markers qPCR Array Kit (GQH-MPM) is designed to facilitate gene expression profiling of 40 marker genes involved in microglial polarization, which has important implications for regulating innate immunological functions. When microglia are stimulated, two potential phenotypes can be observed: pro-inflammatory M1 microglia and anti-inflammatory M2 microglia. The M1 phenotype acts to protect tissues against invading pathogens by releasing cytokines and revoking acute inflammation, while the M2 phenotype acts to restore tissue homeostasis and stimulate tissue repair. Brief examples of how included genes may be grouped according to their functions are shown below:     

·         M1 phenotype marker: TNF, FCGR2A, FCGR2B, FCGR2C, FCGR3A, FCGR3B, CD86, IL1B, NOS2, IL6

·         M2 phenotype marker: MRC1, IL4, IL10, TGFB1, IL1R1, SOCS3, ARG1, IL13, SPHK1

·         Activation/resting markers: CD80, CLEC5A, HEXB, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, IRF8, ITGAM, MYB, PTGES, RUNX1, TLR3, TLR4

·         Additional microglia markers: ADGRE1, AIF1, CD40, CD68, EDA, ITGAM, ITGAX, PTPRC

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

基因查詢™人小膠質(zhì)細(xì)胞偏振標(biāo)志物qPCR陣列板布局*8 個(gè)粗體和斜體控件)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADGRE1

EDA

HLA-DQB1

IL6

PTPRC

β-actin

ADGRE1

EDA

HLA-DQB1

IL6

PTPRC

β-actin

B

AIF1

FCGR2A

HLA-DRA

IRF8

RUNX1

GAPDH

AIF1

FCGR2A

HLA-DRA

IRF8

RUNX1

GAPDH

C

ARG1

FCGR2B

HLA-DRB1

ITGAM

SOCS3

LDHA

ARG1

FCGR2B

HLA-DRB1

ITGAM

SOCS3

LDHA

D

CD40

FCGR2C

IL10

ITGAX

SPHK1

NONO

CD40

FCGR2C

IL10

ITGAX

SPHK1

NONO

E

CD68

FCGR3A

IL13

MRC1

TGFB1

PPIH

CD68

FCGR3A

IL13

MRC1

TGFB1

PPIH

F

CD80

FCGR3B

IL1B

MYB

TLR3

GDC

CD80

FCGR3B

IL1B

MYB

TLR3

GDC

G

CD86

HEXB

IL1R1

NOS2

TLR4

PPC

CD86

HEXB

IL1R1

NOS2

TLR4

PPC

H

CLEC5A

HLA-DQA1

IL4

PTGES

TNF

NTC

CLEC5A

HLA-DQA1

IL4

PTGES

TNF

NTC

 

提示:基因選擇可能會(huì)根據(jù)新的研究和開發(fā)進(jìn)行更新

 

ScienCell基因表達(dá)分析產(chǎn)品訂購信息:

GK001   人類細(xì)胞凋亡qPCR檢測(cè)試劑盒,Human Apoptosis qPCR Array Kit

GK002   人類多能干細(xì)胞qPCR檢測(cè)試劑盒,Human Pluripotent Stem Cell qPCR Array Kit

GK003   人類細(xì)胞周期qPCR檢測(cè)試劑盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測(cè)試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測(cè)試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運(yùn)qPCR檢測(cè)試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人類星型膠質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人類黑素細(xì)胞發(fā)育和色素沉著qPCR檢測(cè)試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質(zhì)細(xì)胞轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測(cè)試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內(nèi)皮細(xì)胞表型異質(zhì)性qPCR檢測(cè)試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內(nèi)皮細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細(xì)胞受體信號(hào)通路qPCR檢測(cè)試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測(cè)試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內(nèi)細(xì)胞分化qPCR檢測(cè)試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應(yīng)力和機(jī)械傳導(dǎo)qPCR檢測(cè)試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號(hào)通路qPCR檢測(cè)試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號(hào)通路qPCR檢測(cè)試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號(hào)靶基因qPCR檢測(cè)試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號(hào)qPCR檢測(cè)試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測(cè)試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應(yīng)qPCR檢測(cè)試劑盒,Human Hypoxia Response qPCR Array Kit

GK036   人類HIF1信號(hào)響應(yīng)qPCR檢測(cè)試劑盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬細(xì)胞細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人類吞噬作用qPCR檢測(cè)試劑盒,Human Phagocytosis qPCR Array Kit

GK039   人類先天免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纖維細(xì)胞生長(zhǎng)因子信號(hào)通路qPCR檢測(cè)試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測(cè)試劑盒,Human Skin Wound Healing qPCR Array Kit

GK056   人體止血qPCR檢測(cè)試劑盒,Human Hemostasis qPCR Array Kit

GK060   人類晝夜節(jié)律qPCR檢測(cè)試劑盒,Human Circadian Rhythm qPCR Array Kit

GK061   人類DNA損傷感應(yīng)qPCR檢測(cè)試劑盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人類細(xì)胞生長(zhǎng)和分裂qPCR檢測(cè)試劑盒,Human Cell Growth and Division qPCR Array Kit

GK063   人細(xì)胞周期蛋白和細(xì)胞周期蛋白依賴性激酶qPCR檢測(cè)試劑盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人類小膠質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人類適應(yīng)性免疫反應(yīng)qPCR檢測(cè)試劑盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人類小膠質(zhì)細(xì)胞Sensome qPCR檢測(cè)試劑盒,Human Microglial Sensome qPCR Array Kit

GK074   人角質(zhì)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR檢測(cè)試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人類周細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Pericyte Biology qPCR Array Kit

GK084   人體軸突導(dǎo)向qPCR檢測(cè)試劑盒,Human Axon Guidance qPCR Array kit

GK087   人類染色質(zhì)組織和重塑qPCR檢測(cè)試劑盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088   VEGF信號(hào)通路qPCR檢測(cè)試劑盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人類Ephrin信號(hào)qPCR檢測(cè)試劑盒,Human Ephrin Signaling qPCR Array Kit

GK090   人體細(xì)胞連接qPCR檢測(cè)試劑盒,Human Cell Junctions qPCR Array Kit

GK091   人肝星狀細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星狀細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人間充質(zhì)至上皮細(xì)胞轉(zhuǎn)化qPCR檢測(cè)試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人類雪旺細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Schwann Cell Biology qPCR Array Kit

GK097   人類平滑肌細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Smooth Muscle Cell Biology qPCR Array Kit

GK098   人類心臟肌細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Cardiac Myocyte Biology qPCR Array Kit

GK099   人類骨骼肌細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Skeletal Muscle Cell Biology qPCR Array Kit

GK100   人角質(zhì)形成細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Keratinocyte Cell Biology qPCR Array Kit

GK101   人類Synoviocyte細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Synoviocyte Cell Biology qPCR Array Kit

GK102   人類滋養(yǎng)層細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Trophoblast Cell Biology qPCR Array Kit

GK103   人前脂肪細(xì)胞生物學(xué)qPCR檢測(cè)試劑盒,Human Preadipocyte Cell Biology qPCR Array Kit

GK104   人類細(xì)胞骨架重塑qPCR檢測(cè)試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit

GK105   人類ECM降解qPCR檢測(cè)試劑盒,Human ECM Degradation qPCR Array Kit

GK106   人類焦點(diǎn)粘附復(fù)合物qPCR檢測(cè)試劑盒,Human Focal Adhesion Complexes qPCR Array Kit

GK107   人內(nèi)皮素信號(hào)qPCR檢測(cè)試劑盒,Human Endothelin Signaling qPCR Array Kit

GK111   人凝集素調(diào)節(jié)免疫應(yīng)答qPCR檢測(cè)試劑盒,Human Lectin Regulation in Immune Response qPCR Array Kit

GK112   人類神經(jīng)分化標(biāo)記qPCR檢測(cè)試劑盒,Human Neural Differentiation Markers qPCR Array Kit

GK113   人類神經(jīng)干細(xì)胞標(biāo)記qPCR檢測(cè)試劑盒,Human Neural Stem Cell Markers qPCR Array Kit

GK114   人類小膠質(zhì)細(xì)胞極化標(biāo)記qPCR檢測(cè)試劑盒,Human Microglial Polarization Markers qPCR Array Kit

GK115   人類骨髓譜系標(biāo)記qPCR檢測(cè)試劑盒,Human Myeloid Lineage Markers qPCR Array Kit

GK116   B細(xì)胞和T細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human B and T Cells Development Markers qPCR Array Kit

GK117   人胸腺細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Thymocyte Development Markers qPCR Array Kit

GK118   人類先天淋巴細(xì)胞發(fā)育標(biāo)記qPCR檢測(cè)試劑盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119   人類神經(jīng)祖細(xì)胞標(biāo)記qPCR檢測(cè)試劑盒,Human Neural Progenitor Markers qPCR Array Kit

GK121   PD-1/PD-L1檢查點(diǎn)途徑qPCR檢測(cè)試劑盒,GeneQuery Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit

GK123   人類生長(zhǎng)因子qPCR檢測(cè)試劑盒,Human Growth Factors qPCR Array Kit

GK124   人類轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Transcription Factors qPCR Array Kit

GK125   人干細(xì)胞轉(zhuǎn)錄因子qPCR檢測(cè)試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細(xì)胞表面標(biāo)記qPCR檢測(cè)試劑盒,Human Cell Surface Markers qPCR Array Kit

GK127   人腦發(fā)育標(biāo)記物qPCR檢測(cè)試劑盒,Human Brain Development Markers qPCR Array Kit

 

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